Figure 9.
Figure 9. PlexinD1 and SH3BP1 play a role in Sema3E induced ECs repulsion. (A) HUVECs transfected with control, PlexinD1, and SH3BP1 siRNA were grown to a confluent monolayer. Cells were photographed 16 h later. HEK293T cells expressing control ligand or Sema3E were prelabeled with Hoechst 33342 and added on top of HUVECs. HEK293T expressing Sema3E (arrows) were surrounded by a cell-free area, whereas HEK293T expressing a control ligand did not exhibit a cell-free area. PlexinD1 and SH3BP1 siRNA-transfected cells did not repel in the presence of a Sema3E source. Bright field and Hoechst 33342 channel are shown. Bar, 200 µm. (B) Quantification of cell free area surrounding HEK293T cells. *, P < 0.01; **, P < 0.001. n = 4. Error bars indicate SEM.

PlexinD1 and SH3BP1 play a role in Sema3E induced ECs repulsion. (A) HUVECs transfected with control, PlexinD1, and SH3BP1 siRNA were grown to a confluent monolayer. Cells were photographed 16 h later. HEK293T cells expressing control ligand or Sema3E were prelabeled with Hoechst 33342 and added on top of HUVECs. HEK293T expressing Sema3E (arrows) were surrounded by a cell-free area, whereas HEK293T expressing a control ligand did not exhibit a cell-free area. PlexinD1 and SH3BP1 siRNA-transfected cells did not repel in the presence of a Sema3E source. Bright field and Hoechst 33342 channel are shown. Bar, 200 µm. (B) Quantification of cell free area surrounding HEK293T cells. *, P < 0.01; **, P < 0.001. n = 4. Error bars indicate SEM.

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