Tension regulation in yeast metaphase spindles. (A) WT and top2-4 spindles, labeled as in Fig. 1. Bars, 1 μm. (B) MSD versus time step size in WT (light blue) and top2-4 (dark blue) cells. (C) Pericentromere stiffness. (D) lacO spot separation distances in WT cells and in top2-4 cells (P < 0.0001, t test). (E) Pericentromere tension distributions are similar between WT and top2-4 cells (P = 0.12, t test). Error bars = SEMs.