Substantial pericentromere stretching tension in budding yeast metaphase. (A) MSD versus time step size in WT cells (W303 strain background). (B) Mean pericentromere stiffness using different treatments. az = azide. (C) Estimating pericentromere rest length using nocodazole (see Materials and methods). Bars, 1 μm. (D) lacO spot separation distances in live cells (n = 390). (E) Pericentromere tension (n = 390). Error bars = SEMs.