The rod region in Myo51 tail determines Myo51 localization. (A) Schematics of Myo51 domains and truncations constructed. (B) Localizations of FL and truncated Myo51. (C and D) Micrographs and quantification of localization of headless Myo51(753–1,471) to the contractile ring in rng8Δ. (E) CoIP assays between Myo51 FL or truncations and Rng9. Arrowheads mark the Myo51 bands with the expected sizes. (F) The Myo51-Myo52 chimera M2IQ2-CC1-GTD2 localizes similarly to FL Myo51 in wt and myo51Δ cells. Cell boundaries are marked with broken lines. Bars, 5 µm.