Rng9 is a binding partner of Rng8. (A) Rng8 and Rng9 coIP with each other (see Materials and methods). (B) Rng8 and Rng9 are interdependent for localization. Bar, 5 µm. (C) FRAP analysis of Rng8 (left) and Rng9 (right) in the contractile ring. Curve fit is shown in red, SD in gray. (D and E) Numbers of Rng8 and Rng9 molecules globally in the whole cell (D) and locally in the contractile ring (E). Error bars indicate 1 SD.