Figure 3.

TRIM9 ubiquitin ligase function is required for Netrin-1–dependent axon branching. E15.5 cortical neurons transfected with Myc or MycTRIM9ΔRING (red) stained with phalloidin to mark F-actin (green) in Netrin-1 and FGF2 branching assays. Arrowheads denote axon branching points. Mean axon branch density ± SEM; n = 593 neurons. P-values were obtained from ANOVA with Tukey’s post-hoc analysis.

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