Role of LIC1-T213 in lysosome dispersal. (A) Rescue of LIC1/LIC2 RNAi-induced lyso/LE dispersal by overexpression of GFP (control), GFP-LIC1 wild type (wt), -T213A, or -T213D in A549 cells shown by immunostaining for GFP (green) and LAMP1 (red). Rescue was observed with GFP-LIC1 wild type and LIC1-T213A but not with LIC1-T213D or GFP. (B) Schematic representation of scoring procedure for lyso/LE dispersal. Cells are scored as strongly dispersed (red), if lysosomes can be detected within the outer, middle, and inner bands. They are scored as partially dispersed (blue) or normal (green) if lysosome localization does not extend beyond to the middle or inner bands, respectively. (C) Quantification of data in A. Mean of three independent experiments with >40 cells each; error bars represent SDs. (D) Role of LIC1-T213 in Ad5-induced lyso/LE dispersal. LIC RNAi rescue with LIC1-T213A reduces Ad5-induced lyso/LE dispersal, in contrast to LIC1 wild-type rescue cells. Ad5 capsid redistribution at 30 min p.i. shows similar virus burden in both cells. Lines depict cell outlines. (E) Quantification of data in D. Mean of three independent experiments with >40 cells each; error bars represent SDs. *, P < 0.05; **, P < 0.01. Bars, 20 µm.