Figure 3.
Figure 3. P4M Golgi localization is independent of Arf1. (A) COS-7 cells expressing mCherry-P4M and PH-FAPP1-GFP before and 20 min after the addition of 5 µg/ml Brefeldin A (BFA). (B) Normalized intensity of PH-FAPP1-GFP and mCherry (mCh)-P4M at the Golgi after treatment with 5 µM Brefeldin A. Data are grand means ± SEM of three independent experiments. Significant (P < 0.05) changes are denoted as the largest p-value over the indicated range (two-way ANOVA; see Materials and methods). (C) COS-7 cells expressing GFP-P4M and ManII1–102–mKusabira Orange before and 20 min after the addition of 5 µg/ml Brefeldin A. (D) Normalized intensity of GFP-P4M and ManII1–102–mKusabira Orange; data are means ± SEM of seven cells from two independent experiments. Bars, 10 µm.

P4M Golgi localization is independent of Arf1. (A) COS-7 cells expressing mCherry-P4M and PH-FAPP1-GFP before and 20 min after the addition of 5 µg/ml Brefeldin A (BFA). (B) Normalized intensity of PH-FAPP1-GFP and mCherry (mCh)-P4M at the Golgi after treatment with 5 µM Brefeldin A. Data are grand means ± SEM of three independent experiments. Significant (P < 0.05) changes are denoted as the largest p-value over the indicated range (two-way ANOVA; see Materials and methods). (C) COS-7 cells expressing GFP-P4M and ManII1–102–mKusabira Orange before and 20 min after the addition of 5 µg/ml Brefeldin A. (D) Normalized intensity of GFP-P4M and ManII1–102–mKusabira Orange; data are means ± SEM of seven cells from two independent experiments. Bars, 10 µm.

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