Figure 1.
Figure 1. Localization of fluorescent protein fusions of the P4M domain (residues 546–647) from L. pneumophila SidM/DrrA protein. (A) GFP-P4M expressed in COS-7 cells and imaged on a spinning-disk confocal; shown are single confocal optical sections along the indicated axes, a 3D maximum intensity projection of 38 optical sections acquired at 0.2-µm intervals, or a color-coded temporal (3Dt) projection of 23 maximum intensity projections acquired at 8.15-s intervals. (B) Colocalization of the iRFP-tagged P4M domain with the PH domains from PLCδ1 and FAPP1. The color-coded images are normalized mean deviation product (nMDP) images of the indicated image pairs, showing the spatial distribution of colocalized pixels. mCh, mCherry. (C) Two P4M domains fused in tandem to GFP are shown as confocal optical sections along the indicated axes. Note the collapsed Golgi morphology. Bars, 10 µm.

Localization of fluorescent protein fusions of the P4M domain (residues 546–647) from L. pneumophila SidM/DrrA protein. (A) GFP-P4M expressed in COS-7 cells and imaged on a spinning-disk confocal; shown are single confocal optical sections along the indicated axes, a 3D maximum intensity projection of 38 optical sections acquired at 0.2-µm intervals, or a color-coded temporal (3Dt) projection of 23 maximum intensity projections acquired at 8.15-s intervals. (B) Colocalization of the iRFP-tagged P4M domain with the PH domains from PLCδ1 and FAPP1. The color-coded images are normalized mean deviation product (nMDP) images of the indicated image pairs, showing the spatial distribution of colocalized pixels. mCh, mCherry. (C) Two P4M domains fused in tandem to GFP are shown as confocal optical sections along the indicated axes. Note the collapsed Golgi morphology. Bars, 10 µm.

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