Figure 1.
Actin organization in the lamella is axially diffraction-limited. (A–C) Actin filaments localized in a U2OS cell by confocal imaging. (A and B) x-y views of projections from below (A) and above (B) the dotted white line in C. Open arrows, open arrowheads, and closed arrowheads denote actin arcs, DSF, and ventral stress fibers, respectively. Brackets denote lamella. (C) Side view of a cell from the region of interest denoted by the broken yellow lines in A and B. The broken white line shows the axially diffraction-limited layer at the bottom of the cell. Bracket denotes the lamella. Bars, 10 µm.