Wnt7a stimulates dispersal of human primary myoblasts. (A) Scratch assay with human myoblasts. Wnt7a treatment significantly increases cell migration into the scar and expression of Rac1-DN prevents this effect. Error bars represent means ± SEM; n = 3. **, P < 0.01. (B) Fzd7-Flag increases human myoblast migration and Rac1-DN prevents this effect. Error bars represent means ± SEM; n = 3. **, P < 0.01. n.s., no significant difference. (C) Strategy used for Wnt7a or vehicle treatment and subsequent transplantation of human primary myoblasts into mdx mice. (D) Number of dystrophin-expressing myofibers after transplantation of Wnt7a- or vehicle-treated cells. Error bars represent means ± SEM; n ≥ 4. ***, P < 0.001. (E) Minimal myofiber Feret of dystrophin-expressing myofibers generated from fusion with Wnt7a- or vehicle-treated human primary myoblasts. Error bars represent means ± SEM; n = 3. *, P < 0.05. (F) Mean maximum cluster difference in muscles transplanted with Wnt7a- or vehicle-treated human primary myoblasts. Error bars represent means ± SEM; n ≥ 4. **, P < 0.01.