UNC-60A (ADF/cofilin) does not regulate other AC polarity and secretion functions. (A) The β-integrin subunit PAT-3::GFP (top, spectral representation of fluorescence intensity) and the netrin receptor UNC-40::GFP (bottom) localized to the invasive cell membrane in wild-type (left, arrowhead) and after loss of unc-60a (right, arrowhead). Note, the integrin indicated by the arrow is PAT-3::GFP that localizes between the central vulval precursor cells (it is not a protrusion from the AC). (B) Lateral- and ventral-view images show the normal deposition of the matrix component hemicentin (shown in grayscale, arrowheads) into the basement membrane below the AC (expressing the F-actin probe mCherry::moeABD, magenta) in wild-type (left) and after loss of unc-60a (right). (C) Vulval induction (indicated by egl-17::GFP, green) was normal after loss of unc-60a, indicating AC (arrowhead) secretion of the ligand LIN-3. (D) Graphs report the polarity of PAT-3::GFP and UNC-40::GFP, and the volume of hemicentin::GFP for wild-type (black) and unc-60a–depleted animals (orange; n ≥ 6 animals; n.s., not significant; Student’s t test; error bars indicate mean ± SEM). (E) UNC-60A drives F-actin disassembly at AC invadopodia, which is required for normal invadopodia dynamics, and the recycling of invadopodial membrane through endolysosome to the plasma membrane.