UNC-60A promotes F-actin disassembly at AC invadopodia. (A) Pre-conversion ACs expressing Dendra2::ACT-1 (a tagged form of the actin monomer found throughout the cytoplasm in monomeric form and incorporated within F-actin at invadopodia; left), immediately post-conversion (converted actin is shown in red and spectrally; middle), and 30 min after conversion (right). Optically highlighted regions of Dendra2::ACT-1 (dotted white circle, arrowhead) began to disperse immediately (asterisks) in wild-type animals (top) and were found throughout the AC by 30 min (arrowheads). In unc-60a (RNAi) animals (bottom), optically highlighted regions of actin failed to disperse, consistent with a lack of F-actin disassembly. (B) The fold enrichment of red signal (converted actin) in the optically highlighted region (dotted white circle) compared with the region outside the highlighted region in the cell is displayed for wild-type and unc-60a (RNAi) animals 30 s after conversion and 30 min after conversion (n = 5 ACs for wild-type animals, n = 4 for unc-60a RNAi animals; **, P = 0.005; Student’s t test; error bars indicate ±SEM. Bar, 5 µm.