UNC-60A promotes F-actin dynamics at AC invadopodia. (A) 3D projections (left) and isosurface renderings (green, right) of the normal F-actin network in wild-type (top; visualized with the F-actin binding probe mCherry::moeABD) and expanded F-actin in a unc-60a (RNAi) animal (bottom; dotted orange line is basement membrane; dotted white line is the AC’s apicolateral membrane). Graph reports the F-actin volume and amount in wild-type (black) and unc-60a (RNAi) animals (gray, n ≥ 10 animals each; P < 0.001, Student’s t test; error bars indicate ±SEM. (B) Ventral-view images (top) show an F-actin–rich AC invadopodium (green, mCherry::moeABD) at the initial basement membrane breach (inset). Bottom panels show a unc-60a (RNAi) animal that has enlarged F-actin foci that fail to invade. (C) Time-series show dynamic F-actin–rich AC invadopodia in a wild-type animal (top) and the enlarged, static F-actin structures in a unc-60a (RNAi) animal (bottom). Bars, 5 µm. (D) Scatterplots report invadopodia lifetime measurements for wild-type (left; invadopodia that were actively breaching the basement membrane are shown on the right) and unc-60a RNAi-treated animals (center). The interquartile range is shown in red; green bars denote the population mean (***, P < 0.0001; Wilcoxon rank-sum test).