Depletion of the dynein regulators NudE/EL delays NE/ER membrane removal from chromatin. (A) Representative cells after depletion of NudE/EL or BICD2 at the indicated times before and after NEBD. Bar, 5 µm. (B) Line blot displaying the progression of NE/ER membrane removal from chromatin upon NudE/EL or BICD2 knockdown (five experiments; means ± SEM; n ≥ 16 per condition). (C) Western blots to confirm efficient knockdown of the indicated proteins. α-Tubulin serves as loading control. IB, immunoblot.