Figure 2.
Figure 2. SUN1/2 are functionally redundant in NE/ER membrane removal. (A) Representative cells after control siRNA treatment or down-regulation of SUN1 and SUN2, either individually or combined, at the indicated time points before and after NEBD. Bar, 5 µm. (B, D, and F) Line blots displaying progression of NE/ER membrane removal from chromatin upon treatment with the indicated siRNAs or with 333 nM of the indicated cytoskeletal drugs (six and five experiments for B and D/F, respectively; means ± SEM; n ≥ 14 per condition). (C and E) Western blots confirming efficient knockdown of SUN1/2 after 72 h of RNAi. β-Actin and α-tubulin serve as loading controls. ctr, control; endog., endogenous; IB, immunoblot.

SUN1/2 are functionally redundant in NE/ER membrane removal. (A) Representative cells after control siRNA treatment or down-regulation of SUN1 and SUN2, either individually or combined, at the indicated time points before and after NEBD. Bar, 5 µm. (B, D, and F) Line blots displaying progression of NE/ER membrane removal from chromatin upon treatment with the indicated siRNAs or with 333 nM of the indicated cytoskeletal drugs (six and five experiments for B and D/F, respectively; means ± SEM; n ≥ 14 per condition). (C and E) Western blots confirming efficient knockdown of SUN1/2 after 72 h of RNAi. β-Actin and α-tubulin serve as loading controls. ctr, control; endog., endogenous; IB, immunoblot.

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