Figure 6.
Figure 6. The microtubule motility and sliding activities of Kip3 are not sufficient to regulate spindle length in the absence of microtubule-destabilizing activity. (a) Representative fluorescence images of Kip3-EYFP and kip3-CC-EYFP on spindles (CFP-tubulin) in anaphase-arrested cells (cdc15-2, 2.5 h at 37°C). (b) Spindle length as a percentage of cell length in cells containing Kip3-EYFP or kip3-CC-EYFP after 2.5 h anaphase arrest. Average ± SE, P < 0.005, n = 90 cells from three experiments for each cell type. Bar, 2 µm.

The microtubule motility and sliding activities of Kip3 are not sufficient to regulate spindle length in the absence of microtubule-destabilizing activity. (a) Representative fluorescence images of Kip3-EYFP and kip3-CC-EYFP on spindles (CFP-tubulin) in anaphase-arrested cells (cdc15-2, 2.5 h at 37°C). (b) Spindle length as a percentage of cell length in cells containing Kip3-EYFP or kip3-CC-EYFP after 2.5 h anaphase arrest. Average ± SE, P < 0.005, n = 90 cells from three experiments for each cell type. Bar, 2 µm.

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