Model of Hok1 in controlling EE motility. (A) Fts1, Fhp1, and Hok1 form a complex that may contain additional, yet unknown, adapter proteins that link dynein and kinesin-3 to this Hok1 complex (b and c). Kinesin-3 binds to Hok1 and, to a lesser extent, directly to EE membranes. (B) Before dynein binding, the Hok1 complex releases kinesin-3. The cargo stops moving but remains attached to the MT. This may reflect the ability of Hok1 to bind MTs, as described for human Hook proteins (Walenta et al., 2001). (C) Dynein leaves the MT plus ends and travels toward the pausing EE, where it interacts with the Hok1 complex. This could involve the dynactin complex, as shown in the fungus A. nidulans (c; Zhang et al., 2011). (D and E) During pausing and while dynein moves the cargo to minus ends of MTs, kinesin-3 rebinds to the Hok1 complex. Speculative parts of the model are indicated by question marks.