Figure 3.
Figure 3. Colocalization of HookA-GFP signals with mCherry-RabA–labeled early endosomes. (A) Images of HookA-GFP and mCherry-RabA in the same cell. (B) Kymographs of the GFP and mCherry signals obtained via duel-view imaging. Arrows are shown to indicate that some HookA-GFP signals are associated with motile early endosomes. (C) HookA-GFP and mCherry-RabA in the ΔkinA mutant. HookA-GFP signals were concentrated at every hyphal tip where early endosomes accumulate (n = 50). (D) HookA-GFP and mCherry-RabA in the Δp25 mutant. HookA-GFP signals were concentrated at every hyphal tip where early endosomes accumulate (n = 50). The same minimal medium containing 1% glycerol as a carbon source was used for cells shown in A, C, and D. For the medium used for growing the cells viewed by the dual-view imaging (B), 0.1% fructose instead of 1% glycerol was used as a carbon source to reduce the intensity of the mCherry signals. Bars, 5 µm.

Colocalization of HookA-GFP signals with mCherry-RabA–labeled early endosomes. (A) Images of HookA-GFP and mCherry-RabA in the same cell. (B) Kymographs of the GFP and mCherry signals obtained via duel-view imaging. Arrows are shown to indicate that some HookA-GFP signals are associated with motile early endosomes. (C) HookA-GFP and mCherry-RabA in the ΔkinA mutant. HookA-GFP signals were concentrated at every hyphal tip where early endosomes accumulate (n = 50). (D) HookA-GFP and mCherry-RabA in the Δp25 mutant. HookA-GFP signals were concentrated at every hyphal tip where early endosomes accumulate (n = 50). The same minimal medium containing 1% glycerol as a carbon source was used for cells shown in A, C, and D. For the medium used for growing the cells viewed by the dual-view imaging (B), 0.1% fructose instead of 1% glycerol was used as a carbon source to reduce the intensity of the mCherry signals. Bars, 5 µm.

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