Figure 1.
Figure 1. Phenotype of the eedA1 mutant and rescue of the mutant phenotype by the gene encoding HookA. (A) A schematic diagram depicting the phenotype of the Δp25 mutant in comparison to the ΔnudA (dynein HC) mutant. Note that early endosomes abnormally accumulate at the hyphal tip in both the ΔnudA and Δp25 mutants but a nuclear distribution phenotype is shown only in the ΔnudA mutant. Red, early endosomes. Dark blue, nuclei. Black lines, microtubules. (B) A brief outline of the mutant-screening procedure. (C) Colony phenotypes of the eedA1 mutant and a wild-type strain. (D) Microscopic images showing the distributions of mCherry-RabA–labeled early endosomes (mCherry-RabA) and GFP-labeled dynein HC (GFP-HC). The same cells are shown for both the mCherry-RabA and GFP-HC images. Although bidirectional movements of mCherry-RabA–labeled early endosomes are not completely abolished, ∼83% of eedA1 hyphal tips show obvious accumulation of mCherry-RabA signals (n = 140), whereas none of the wild-type hyphal tips show this accumulation (n = 100). Dynein comets are present in all wild-type and mutant cells. (E) Images of nuclei stained by a DNA dye, DAPI, in wild type and the eedA1 mutant. The pattern of nuclear distribution in the eedA1 mutant is normal, as none of the mutant cells show any cluster of four or more nuclei when grown under the same conditions that allow us to see the hyphal tip mCherry-RabA accumulation (n > 100 for wild type, and n > 100 for the mutant). (F) Rescue of the eedA1 mutant phenotype with the HookA-encoding DNA. (left) Colony phenotypes of the eedA1 mutant, eedA1 mutant transformed with the HookA-encoding gene, and a wild-type strain. (right) Distribution of mCherry-RabA–labeled early endosomes in an eedA1 mutant transformed with the HookA-encoding gene. None of the hyphal tips show accumulation of mCherry-RabA signals (n = 30). Bars, 5 µm.

Phenotype of the eedA1 mutant and rescue of the mutant phenotype by the gene encoding HookA. (A) A schematic diagram depicting the phenotype of the Δp25 mutant in comparison to the ΔnudA (dynein HC) mutant. Note that early endosomes abnormally accumulate at the hyphal tip in both the ΔnudA and Δp25 mutants but a nuclear distribution phenotype is shown only in the ΔnudA mutant. Red, early endosomes. Dark blue, nuclei. Black lines, microtubules. (B) A brief outline of the mutant-screening procedure. (C) Colony phenotypes of the eedA1 mutant and a wild-type strain. (D) Microscopic images showing the distributions of mCherry-RabA–labeled early endosomes (mCherry-RabA) and GFP-labeled dynein HC (GFP-HC). The same cells are shown for both the mCherry-RabA and GFP-HC images. Although bidirectional movements of mCherry-RabA–labeled early endosomes are not completely abolished, ∼83% of eedA1 hyphal tips show obvious accumulation of mCherry-RabA signals (n = 140), whereas none of the wild-type hyphal tips show this accumulation (n = 100). Dynein comets are present in all wild-type and mutant cells. (E) Images of nuclei stained by a DNA dye, DAPI, in wild type and the eedA1 mutant. The pattern of nuclear distribution in the eedA1 mutant is normal, as none of the mutant cells show any cluster of four or more nuclei when grown under the same conditions that allow us to see the hyphal tip mCherry-RabA accumulation (n > 100 for wild type, and n > 100 for the mutant). (F) Rescue of the eedA1 mutant phenotype with the HookA-encoding DNA. (left) Colony phenotypes of the eedA1 mutant, eedA1 mutant transformed with the HookA-encoding gene, and a wild-type strain. (right) Distribution of mCherry-RabA–labeled early endosomes in an eedA1 mutant transformed with the HookA-encoding gene. None of the hyphal tips show accumulation of mCherry-RabA signals (n = 30). Bars, 5 µm.

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