Figure 4. Behavior of type 2 nodes... | Rockefeller University Press

Figure 4.

$Figure 4. Behavior of type 2 nodes marked with Blt1p-mEGFP in cdr2Δ cells lacking type 1 nodes. (A) MSDs versus time step of Blt1p-mEGFP nodes in cdr2Δ cells. Symbols: five type 2 nodes with intensity <12,000 A.U. (open triangles); three Blt1p-mEGFP particles with fluorescence intensity >12,000 A.U. (closed triangles), likely aggregates of nodes. (B) Plot of diffusion coefficients of Blt1p-mEGFP nodes in cdr2Δ cells during early interphase as a function of their fractional distance from the new end of the cell. Symbols: nodes with intensity <12,000 A.U. (open triangles); particles with intensity ≥12,000 A.U. (closed triangles). (C) Pairs of micrographs with a negative contrast sum intensity projection image of three 300-nm z slices (left) and a temporal color projection image at 2-s intervals for 200 s in early and late interphase cells expressing Blt1p-mEGFP in a cdr2Δ strain (right). Many more equatorial nodes were mobile in early G2 than G2/M (also see Fig. S4). Dotted ovals outline cells. (D) Time series of reverse contrast maximum intensity projections of seven z sections taken at 600-nm steps showing the assembly of a contractile ring in a cdr2Δ cell expressing Blt1p-mEGFP. See Fig. S4 for higher time resolution. (E) Outcome plots of the time course of contractile ring assembly and constriction in cells expressing type 2 node protein Blt1p-mEGFP ±Cdr2p. Symbols: wild-type cdr2+ cells (closed circles and squares); cdr2Δ cells (open circles and squares); fraction of cells with a compact contractile ring (circles); and fraction of cells with a constricting contractile ring (squares). Time is in minutes from SPB separation. Bars, 2 µm.$

Behavior of type 2 nodes marked with Blt1p-mEGFP in cdr2Δ cells lacking type 1 nodes. (A) MSDs versus time step of Blt1p-mEGFP nodes in cdr2Δ cells. Symbols: five type 2 nodes with intensity <12,000 A.U. (open triangles); three Blt1p-mEGFP particles with fluorescence intensity >12,000 A.U. (closed triangles), likely aggregates of nodes. (B) Plot of diffusion coefficients of Blt1p-mEGFP nodes in cdr2Δ cells during early interphase as a function of their fractional distance from the new end of the cell. Symbols: nodes with intensity <12,000 A.U. (open triangles); particles with intensity ≥12,000 A.U. (closed triangles). (C) Pairs of micrographs with a negative contrast sum intensity projection image of three 300-nm z slices (left) and a temporal color projection image at 2-s intervals for 200 s in early and late interphase cells expressing Blt1p-mEGFP in a cdr2Δ strain (right). Many more equatorial nodes were mobile in early G2 than G2/M (also see Fig. S4). Dotted ovals outline cells. (D) Time series of reverse contrast maximum intensity projections of seven z sections taken at 600-nm steps showing the assembly of a contractile ring in a cdr2Δ cell expressing Blt1p-mEGFP. See Fig. S4 for higher time resolution. (E) Outcome plots of the time course of contractile ring assembly and constriction in cells expressing type 2 node protein Blt1p-mEGFP ±Cdr2p. Symbols: wild-type cdr2⁺ cells (closed circles and squares); cdr2Δ cells (open circles and squares); fraction of cells with a compact contractile ring (circles); and fraction of cells with a constricting contractile ring (squares). Time is in minutes from SPB separation. Bars, 2 µm.