Phosphorylation of intermediate chain IC138 of IDA subspecies f. TCA-fixed axonemal proteins were separated on a 5% acrylamide gel and transferred to a polyvinylidene difluoride membrane. The blot was probed with IC138 antiserum. Hyperphosphorylation of IC138 was observed in pf14, pf6, pf6 rsp4CHA, pf6 rsp4C, pf6 rsp4CGFP, cpc1, and cpc1 RSP4C, and intermediate phosphorylation was observed in rsp4C. The broken line shows the height of the wild-type (WT) band.