Pex19 overproduction and PEX19 or PEX25 deletion. (A and B) WB analysis (A) and protein quantification (B) in WT (lane 1; blue), pex3 atg1 (lane 2; red), and pex3 atg1-P_AOXPEX19 (lane 3; green) cells grown for 6 h on MM-M/G. The protein levels in WT were set to 100%. (C and D) FM images (C) and quantification (D) of Pmp47-mGFP and Pex10-mGFP in pex3 atg1 (1; red) and pex3 atg1-P_AOXPex19 (2; green) cells grown for 6 h on MM-M/G. Control cells in D did not produce mGFP. Significance indications: n.s., P < 0.10; *, 0.10 > P > 0.05; **, 0.05 > P > 0.01; ***, P < 0.01. Error bars indicate SD. (E and F) iEM analysis of pex19 (E) and pex19 atg1 (F) cells using α-Pex14 antibodies. (G) EM analysis of KMnO₄-fixed pex3 atg1 pex25 cells grown for 16 h on MM-M/G–showing membrane vesicles (arrows). (H) FM image of pex3 atg1 pex25 cells producing Pex14-mGFP. CW, cell wall; M, mitochondrion; N, nucleus; V, vacuole. Bars: (C) 2 µm; (E and F) 100 nm; (G) 250 nm; (H) 2.5 µm.