Figure 4.
Figure 4. Mvp1 possesses potent membrane remodeling activity. (A) Gallery of micrographs of negative-stained liposomes incubated with SNX-BAR Mvp1 at indicated concentrations. Bar, 200 nm. Montages of micrographs for each condition showing representative vesicles or tubules generated by Mvp1 are shown. (B) Histogram of the diameter of vesicles generated by Mvp1 (n = 129 vesicles) in 5-nm bins. (C) Box-plot compares the distribution of vesicle diameters generated by Mvp1. Average vesicle diameters of liposomes alone are 171.6 ± 96.8 nm, n = 55, and with Mvp1 are 54.4 ± 12.3 nm, P < 0.0001 (unpaired t test). (D) Histogram of the diameter of tubules generated by 10 or 20 µM Mvp1 in 5-nm bins. 10 µM Mvp1 (n = 29) generated average tubule diameters of 63.3 ± 15.3 nm, and 20 µM Mvp1 (n = 9) generated average tubule diameters of 35.8 ± 18.2 nm, P < 0.0001 (unpaired t test).

Mvp1 possesses potent membrane remodeling activity. (A) Gallery of micrographs of negative-stained liposomes incubated with SNX-BAR Mvp1 at indicated concentrations. Bar, 200 nm. Montages of micrographs for each condition showing representative vesicles or tubules generated by Mvp1 are shown. (B) Histogram of the diameter of vesicles generated by Mvp1 (n = 129 vesicles) in 5-nm bins. (C) Box-plot compares the distribution of vesicle diameters generated by Mvp1. Average vesicle diameters of liposomes alone are 171.6 ± 96.8 nm, n = 55, and with Mvp1 are 54.4 ± 12.3 nm, P < 0.0001 (unpaired t test). (D) Histogram of the diameter of tubules generated by 10 or 20 µM Mvp1 in 5-nm bins. 10 µM Mvp1 (n = 29) generated average tubule diameters of 63.3 ± 15.3 nm, and 20 µM Mvp1 (n = 9) generated average tubule diameters of 35.8 ± 18.2 nm, P < 0.0001 (unpaired t test).

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