Mvp1 and Vps17 retromer SNX-BAR coat tubules that bud from the endosome. (A) Micrographs of Mvp1-GFP–, Vps17-GFP–, or Vps26-GFP–decorated endosomes captured by time-lapse fluorescence deconvolution microscopy. Arrowheads point to a tubule of interest and open arrows (<) indicate a presumptive fission event. Images were captured at a single focal plane within cells and acquired at 270-ms intervals. (B) Micrographs showing tubules emanating from Vps17-GFP– and Mvp1-2xmRFP–decorated endosomes were captured by time-lapse fluorescence microscopy. An example of a tubule decorated by both Vps17-GFP and Mvp1-2xmRFP (“Mixed”) is shown on the top, a tubule decorated only by Vps17-GFP in the middle, and a tubule decorated by only Mvp1-GFP on the bottom. (C) Quantitation of the different types of SNX-BAR–coated tubules. (D) Micrographs showing Vps17-GFP–coated (top) or Mvp1-GFP– coated (bottom) tubules emanating from an endosome also decorated by Did2-mKate2. Image capture speeds for each strain was 50 ms for the GFP channel and 200 ms for the RFP channel. Bar, 500 nm.