Figure 3.
Figure 3. PIP3 patches organize macropinosomes. (A and B) Confocal series showing turnover of PIP3 patches in NC4 and axenically grown AX2 cells expressing the PIP3 marker PH-CRAC-GFP. Arrowheads mark budding. Bar, 10 µm. (C) Confocal series showing protrusions in an axenically cultivated AX2 cell expressing PH-CRAC-GFP and the F-actin marker Lifeact-mRFP. Arrows indicate negative curvature. Bar, 5 µm. This corresponds with Video 1. (D) Kymograph of a line drawn through the protrusion in C.

PIP3 patches organize macropinosomes. (A and B) Confocal series showing turnover of PIP3 patches in NC4 and axenically grown AX2 cells expressing the PIP3 marker PH-CRAC-GFP. Arrowheads mark budding. Bar, 10 µm. (C) Confocal series showing protrusions in an axenically cultivated AX2 cell expressing PH-CRAC-GFP and the F-actin marker Lifeact-mRFP. Arrows indicate negative curvature. Bar, 5 µm. This corresponds with Video 1. (D) Kymograph of a line drawn through the protrusion in C.

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