Figure 6.
Figure 6. M1–AQP4, but not M23–AQP4, enhances cell migration and increases lamellipodial stability. (A) Phase-contrast images of Coomassie-stained U87-MG cells (nontransfected or stably expressing M1– or M23–AQP4) after migration through 8-µm pore Transwells in the presence of a 1–1% or 1–10% FBS gradient. (B) Percentage of cells that migrated across Transwells under each condition. Bars indicate mean ± SE, n = 3 each; *, P < 0.05 by t test. (C) Phase-contrast images (left) and kymographs (right) of astrocytes of the indicated genotype migrating into a scratch wound generated by re-slicing 30-min video sequences along the indicated lines. (D) Mean lamellipodial extension duration and distance in kymographs from 5–8 cells for each condition. Bars indicate mean ± SE; *, P < 0.05 vs. AQP4+/+ by t test.

M1–AQP4, but not M23–AQP4, enhances cell migration and increases lamellipodial stability. (A) Phase-contrast images of Coomassie-stained U87-MG cells (nontransfected or stably expressing M1– or M23–AQP4) after migration through 8-µm pore Transwells in the presence of a 1–1% or 1–10% FBS gradient. (B) Percentage of cells that migrated across Transwells under each condition. Bars indicate mean ± SE, n = 3 each; *, P < 0.05 by t test. (C) Phase-contrast images (left) and kymographs (right) of astrocytes of the indicated genotype migrating into a scratch wound generated by re-slicing 30-min video sequences along the indicated lines. (D) Mean lamellipodial extension duration and distance in kymographs from 5–8 cells for each condition. Bars indicate mean ± SE; *, P < 0.05 vs. AQP4+/+ by t test.

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