![Figure 5. Shroom increases myosin II cortical localization and planar polarity during convergent extension. (A–C) Myo:GFP in time-lapse videos of wild-type (WT; A), ShrmΔ11 mutant (B), and ShrmA RNAi (C) embryos (t = 0 is the onset of elongation; stage 7 [0–10 min]; stage 8 [10–35 min]). Anterior is left, and ventral is down. Bar, 10 µm. (D) Junctional enrichment is the ratio of the mean pixel intensity at adherens junctions divided by the mean pixel intensity in the medial–apical cortex and cytoplasm (pixels ≥1 µm from the cell boundary). AP intensity is the absolute AP edge intensity normalized to the mean pixel intensity for the image. Planar polarity is the mean intensity of AP edges (75–90° relative to the AP axis) divided by the mean intensity of DV edges (0–15°). Myo:GFP intensity was quantified directly in living embryos. (E–G) Myo:GFP junctional enrichment (E), AP intensity (F), and planar polarity (G) in time-lapse videos of wild-type (gray), ShrmA RNAi (blue), and ShrmΔ11 mutant (red) embryos. (E) Myo:GFP junctional enrichment was significantly reduced in Shroom mutants (P < 0.001 at 15 and 30 min) but did not reach statistical significance for ShrmA RNAi. (F) Myo:GFP AP intensity was significantly reduced in Shroom mutant and ShrmA RNAi embryos (P ≤ 0.003 at 15 and 30 min). (G) Myo:GFP planar polarity was significantly reduced in Shroom mutant and ShrmA RNAi embryos (P ≤ 0.02 at 15 and 30 min). A single value was obtained for each image by analyzing 100–200 edges/time point, and four time points/video in three wild-type, seven ShrmΔ11 mutant, and four ShrmA RNAi videos were analyzed. *, P ≤ 0.02. Means ± SEM between images are shown.](https://cdn.rupress.org/rup/content_public/journal/jcb/204/4/10.1083_jcb.201307070/6/jcb_201307070_fig5.jpeg?Expires=1771680914&Signature=f3iYfVwjinYEisxbTz7pj-G5YKw-~8OByMqHicrhR4qmmntrbWE-KECpNEAGD2~0RvphSWlAVlotNZ32VHwm6kO1HZwraU~kmogXltqeh3z5l0VxUe8mJMrPs0oYZbbmjMarjUC6QiTlB2b-4xOviMJTAwNru06WcnYq-Gksj0-BQjqhwZUeNpeoj8Cmc6An4OJDQnIHdLoEZ4XA6KxrU8ny0cRAHltXiqhMTmFTATSiJAxgQQWU9RJK2cMfj4I2CSVjaNaF0CA5VFHrqsSEOXVFy9I0xjRozHRRT0TAK6ROUfvES5g56UA4YUf9toKVH9ztpTXJZ86opmK~hIqDbw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Shroom increases myosin II cortical localization and planar polarity during convergent extension. (A–C) Myo:GFP in time-lapse videos of wild-type (WT; A), ShrmΔ11 mutant (B), and ShrmA RNAi (C) embryos (t = 0 is the onset of elongation; stage 7 [0–10 min]; stage 8 [10–35 min]). Anterior is left, and ventral is down. Bar, 10 µm. (D) Junctional enrichment is the ratio of the mean pixel intensity at adherens junctions divided by the mean pixel intensity in the medial–apical cortex and cytoplasm (pixels ≥1 µm from the cell boundary). AP intensity is the absolute AP edge intensity normalized to the mean pixel intensity for the image. Planar polarity is the mean intensity of AP edges (75–90° relative to the AP axis) divided by the mean intensity of DV edges (0–15°). Myo:GFP intensity was quantified directly in living embryos. (E–G) Myo:GFP junctional enrichment (E), AP intensity (F), and planar polarity (G) in time-lapse videos of wild-type (gray), ShrmA RNAi (blue), and ShrmΔ11 mutant (red) embryos. (E) Myo:GFP junctional enrichment was significantly reduced in Shroom mutants (P < 0.001 at 15 and 30 min) but did not reach statistical significance for ShrmA RNAi. (F) Myo:GFP AP intensity was significantly reduced in Shroom mutant and ShrmA RNAi embryos (P ≤ 0.003 at 15 and 30 min). (G) Myo:GFP planar polarity was significantly reduced in Shroom mutant and ShrmA RNAi embryos (P ≤ 0.02 at 15 and 30 min). A single value was obtained for each image by analyzing 100–200 edges/time point, and four time points/video in three wild-type, seven ShrmΔ11 mutant, and four ShrmA RNAi videos were analyzed. *, P ≤ 0.02. Means ± SEM between images are shown.
