Figure 3.
Figure 3. The Shroom actin-binding protein is planar polarized in intercalating cells. (A and B) Localization of Shroom (A–B) and Baz/Par-3 (A′ and B) in wild-type embryos at the indicated stages. (A) Anterior is left, and ventral is down. (B) Cross section of a stage 8 embryo, with apical up. (C) Planar polarized enrichment of Shroom at AP cell boundaries (75–90° with respect to the AP axis) relative to DV cell boundaries (0–15°). (D) Planar polarized enrichment of Par-3 at DV cell boundaries relative to AP cell boundaries at the indicated stages. Note that Shroom planar polarity appears later than Par-3. A single value was obtained for each image by averaging 100–200 edges/image; 5–13 images in 2–7 embryos were analyzed/stage, except early stage 6 (three images in one embryo). Means ± SEM between images are shown. Bars, 10 µm.

The Shroom actin-binding protein is planar polarized in intercalating cells. (A and B) Localization of Shroom (A–B) and Baz/Par-3 (A′ and B) in wild-type embryos at the indicated stages. (A) Anterior is left, and ventral is down. (B) Cross section of a stage 8 embryo, with apical up. (C) Planar polarized enrichment of Shroom at AP cell boundaries (75–90° with respect to the AP axis) relative to DV cell boundaries (0–15°). (D) Planar polarized enrichment of Par-3 at DV cell boundaries relative to AP cell boundaries at the indicated stages. Note that Shroom planar polarity appears later than Par-3. A single value was obtained for each image by averaging 100–200 edges/image; 5–13 images in 2–7 embryos were analyzed/stage, except early stage 6 (three images in one embryo). Means ± SEM between images are shown. Bars, 10 µm.

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