Ribosome distribution in U. maydis. (A) Organization of a U. maydis hyphal cell. The cell expands at its apex (“Growing tip”), while vacuolated sections are separated by the septum. The nucleus is located near the center. (B) Electron micrograph of the apical region of a hypha. Left inset shows cytoplasmic ribosomes. Various organelles are encircled by red dotted lines. Bar on the left, 0.25 µm. (C) False-colored images of Rpl25-GFP– and Rps3-mCherry3–expressing hyphal cells. The fluorescent signal is evenly distributed over most parts of the cell, but reduced near the cell tip. The intensity color code is given in the lower left. Images were 2D deconvolved using the software MetaMorph and adjusted in brightness, contrast, and gamma settings. (D) Intensity profile of Rpl25-GFP along hyphal cells. Each data point represents the mean ± SEM (error bars); n = 10 cells from a single representative experiment. Ribosomes are evenly distributed along most parts of the cell, but partially excluded from the organelle rich tip.
