Roles of Ena, Dia, and CP in regulating filopodia and broad protrusions. (A and B) Quantification of filopodia number, length, and broad protrusion activity (V_cr = 4 pixels/frame) of cells in overexpression and knockdown backgrounds. Numbers of cells used for analysis were actin n = 16, DiaΔDAD n = 34, Ena overexpression (OE) n = 31, SK knockdown (KD) n = 21, CP knockdown n = 9, Dia knockdown n = 10, Ena knockdown n = 10, Ena knockdown DiaΔDAD n = 7, and Dia knockdown Ena overexpression n = 10. Error bars show 95% confidence interval (mean ± two standard errors).