FiloTrack module for detecting and tracking filopodia. (A and B) Screenshots of GUI. The GUI contains a view of the cell, analysis results, and control panels for adjusting parameters and viewing results. (A) Filopodia detection. Cell data from BisectoGraph are loaded using the File menu, and the cell boundary appears in the cell view section (top left). Checking Show BisectoGraph and Activate Filopodia Detection displays the cell MAT and identified filopodia. Red arrows point to scroll bars to select movie frame (top), critical width (middle), and critical length (bottom). Filopodia meeting specified values of R_cr and L_cr are shown in red. Protrusions not meeting these criteria are shown in yellow. The number of protrusions, mean length, and cell perimeter for the current frame are highlighted by the red box. The Show Results button loads results for all time frames to the results section (top right) where filopodia number (top subsection) and mean length ± SD (bottom subsection) are shown. (B) Filopodia tracking. Clicking Activate Filopodia Tracking and Show Results changes the results section to show all time lines of the detected filopodia (vertical green lines). The magenta arrow marks one of the empty circles indicating temporal gaps in filopodia tracks. The red arrowhead indicates the scroll bar to select a single filopodium (yellow line in results section and yellow dots in cell view [black arrows]). The red box highlights lifetime information for the selected filopodium. Red arrows point to scroll bars for easily changing distance, gap filling, and lifetime filter parameters, with cell view and results sections updating accordingly.