CellGeo platform architecture and qualitative interpretation of the MAT. (A) CellGeo package pipeline for defining, detecting and tracking both thin or broad cellular protrusions or growth cones. (B) Drosophila D16C3 cell expressing GFP-actin with four kymographs labeled 1–4 show high variability of protrusiveness within a single cell, making analysis biased by placement. Bar, 5 µm. (C) A cell and its boundary (yellow). Bar, 10 µm. (D) Distance function F(x, y), which measures the minimum distance from each point (x, y) inside the cell to the cell boundary. (E) Ridges (surface discontinuities) of the minimum distance function in D. (F) Medial axis as a top view of the ridges in E.