Figure 1.
TNF and IL-1 induce the accumulation of NEMO in punctate structures. Immunofluorescence analysis of NEMO in U20S cells left untreated or stimulated with IL-1 (A) or TNF (B) for 12 min. In the two right-hand images of A and in B, the cells were subjected to saponin extraction before fixation. The insets in the images show magnified areas (indicated by the boxed regions). Bars, 5 µm.