Talpid3 forms a ring-like structure at the distal rim of both mother and daughter centrioles. (A and B) RPE1 cells were visualized using an OMX super-resolution microscope in asynchronously growing conditions stained with the indicated combinations of antibodies. (C–E) Antibodies against glutamylated tubulin (GT335), Cep164, and Talpid3_2 were used to stain RPE1 cells during growing (C), 6 h serum starvation (D), and 24 h serum starvation (E) conditions. The bottom panels of C–E show y-axis rotations of the top panels by 165° (C), 48° (D), and 150° (E) in a clockwise manner to best represent the sub-centriolar localization pattern of Talpid3. (F) Schematic representation of the centrosome illustrates the localization of Talpid3 at the distal rim of both the mother centrioles (MC) and daughter centrioles (DC). Bars, 500 nm.