Figure 3.
Figure 3. Arp2/3 depletion or inhibition activates the canonical NF-κB pathway. (A) Blot showing phospho-p65 (Ser536; p-p65), total p65, and GAPDH levels in NS and 2×KD cells. (B) Blot showing p-p65, total p65, and GAPDH levels in NS cells treated with 100 µM CK666 or its inactive control CK689 for 6 h (right). (C, top) Representative immunofluorescent staining showing p-p65 localization in wild-type (WT) and 2×KD cells. (bottom) Percentages of cells having nuclear p-p65 were quantified and graphed. (D) Blot showing p-p65, p65, and GAPDH levels in 2×KD cells treated with an IKK inhibitor cmpdA for the indicated times. Fold changes in band intensity averaged across the three experiments (normalized to loading control and experimental control) are shown below each band.

Arp2/3 depletion or inhibition activates the canonical NF-κB pathway. (A) Blot showing phospho-p65 (Ser536; p-p65), total p65, and GAPDH levels in NS and 2×KD cells. (B) Blot showing p-p65, total p65, and GAPDH levels in NS cells treated with 100 µM CK666 or its inactive control CK689 for 6 h (right). (C, top) Representative immunofluorescent staining showing p-p65 localization in wild-type (WT) and 2×KD cells. (bottom) Percentages of cells having nuclear p-p65 were quantified and graphed. (D) Blot showing p-p65, p65, and GAPDH levels in 2×KD cells treated with an IKK inhibitor cmpdA for the indicated times. Fold changes in band intensity averaged across the three experiments (normalized to loading control and experimental control) are shown below each band.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal