Arp2/3-depleted cells show increased expression of SASP pathway genes. (A) The transcriptomes of IA32 control fibroblasts and their Arp2/3-depleted derivatives (2×KD) were analyzed by RNA-Seq. Representative genes of the serum response factor and SASP pathways selected from the RNA-Seq data (Table S1) are shown. Fold change represents the fold increase in expression (RPKM) for 2×KD cells from control cells, with negative numbers representing the fold decrease in expression. (B) DAVID analysis of the top 500 genes (>10-fold up-regulated) with the highest fold increase in 2×KD cells, showing overrepresented gene groups as ranked by Benjamini score. (C) qRT-PCR showing fold changes of a subset of cytokines, growth factors, and matrix metalloproteinases up-regulated in 2×KD cells in the RNA-Seq. Two biological replicates (three technical replicates each) for each sample are shown. (D) ELISA showing levels of secreted IL-6, MMP3, and HGF in NS and 2×KD cells. Error bars show 95% confidence interval (CI). ****, P < 0.001; **, P < 0.01 by Student’s t test.