CLASP2 is required to maintain the proper dynamics and functionality of AJs. (A) Graph showing the fluorescence recovery after photobleaching of p120-cherry in a ROI selected at an area of cell–cell contact, in both scramble and CLASP2 knockdown (KD) mKer. Dots represent arithmetic means ± SEM, and solid lines are best-fit single exponential curves. Data were normalized to prebleach and postbleach values. (B) CLASP2-deficient mKer and their WT counterparts (scramble) were allowed to attach to Fc-ECad–coated plates for 30 min and 1 h. After the removal of nonadhered cells, the number of cells attached to the plates was quantified in several fields (n = 3 independent experiments, 40 images per experiment). As negative controls, plates coated with mouse Fc and mKer in the presence of EDTA were used. Data are represented as means ± SEM; ***, P < 0.0001, Mann–Whitney U test. (C) CLASP2-deficient mKer and their WT counterparts (scramble) were treated for 6 h with calcium to induce AJ formation. Calcium was removed from the cell culture media to induce AJ disassembly, and 30 min after, cells were fixed and immunostained for ECad, CLASP2, and α-tubulin. Insets are magnifications of the boxed regions. Bars: (main images) 25 µm; (insets) 5 µm.