Figure 4.
Figure 4. Cellular CP110 levels influence centriole length. (A–M) Panels show representative EM images of centrioles in larval wing disc cells of various genotypes (as indicated on each panel); n (total centrioles; total wing discs): 134;9 (A), 127;9 (B), 45;4 (C), 53;3 (D), 61;5 (E), 75;5 (F), 109;9 (G), 48;3 (H), 98;8 (I), 87;7 (J), 75;5 (K), 42;4 (L), and 93;7 (M). Arrows highlight MT extensions. (N and O) Bar charts show the quantification of centriole length in these genotypes. Note that the overexpression of CP110S-GFP dramatically shortens centrioles in a WT background, but has a less marked effect in the CP110Δ background (only bordering on the statistically significant). We suspect that this might be because MT extensions are still present in CP110Δ cells overexpressing CP110S-GFP, potentially contributing to the slight lengthening of the centriole. Bar, 100 nm.

Cellular CP110 levels influence centriole length. (A–M) Panels show representative EM images of centrioles in larval wing disc cells of various genotypes (as indicated on each panel); n (total centrioles; total wing discs): 134;9 (A), 127;9 (B), 45;4 (C), 53;3 (D), 61;5 (E), 75;5 (F), 109;9 (G), 48;3 (H), 98;8 (I), 87;7 (J), 75;5 (K), 42;4 (L), and 93;7 (M). Arrows highlight MT extensions. (N and O) Bar charts show the quantification of centriole length in these genotypes. Note that the overexpression of CP110S-GFP dramatically shortens centrioles in a WT background, but has a less marked effect in the CP110Δ background (only bordering on the statistically significant). We suspect that this might be because MT extensions are still present in CP110Δ cells overexpressing CP110S-GFP, potentially contributing to the slight lengthening of the centriole. Bar, 100 nm.

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