Centriolar MTs are dramatically elongated in somatic cells that lack CP110. (A–D) Panels show projections of an EM tomogram of a centriole in a WT (A and C) and a CP110Δ (B and D) larval wing disc cell (A and B) or brain cell (C and D). The centriolar MTs extend dramatically (arrows) beyond the centriole (brackets) in CP110Δ cells (see Video 1 for representative tomograms). (E–H) Quantification of the centriolar MT extensions in WT (E), CP110Δ (F), CP110Δ rescued by CP110L-GFP (G), and CP110Δ rescued by CP110S-GFP (H) wing disc cells. Panels show representative EM images, and the percentage of centrioles with clearly elongated centriolar MTs is indicated. n (total centrioles; total wing discs): 134;9 (E), 127;9 (F), 45;4 (G), and 53;3 (H). (I–M) Centrioles in third instar larval brains from CP110Δ flies expressing various centriolar-GFP markers (as indicated) were stained for GFP (blue), for acetylated tubulin (red, to reveal the centriolar MT extensions), and various other centriolar proteins (green, as indicated). Bars: (A–H) 100 nm; (I–M) 1 µm.