Figure 1. PAN of noncentrosomal MTs... | Rockefeller University Press

Figure 1.

$Figure 1. PAN of noncentrosomal MTs associate with the cell–cell junction in a side-by-side fashion. (A) SIM images of tubulin immunofluorescence in the apical and subapical planes of Eph4 cells. (B) Schematic drawing of the noncentrosomal MTs in epithelial cell sheets. In addition to the conventional noncentrosomal MTs, which are directed along the apicobasal axis, the PAN of noncentrosomal MTs appeared in the most apical plane of epithelial cell sheets. (C) SIM images of tubulin immunofluorescence in Eph4 cells. The planar apical noncentrosomal MTs are laterally associated with the cell–cell adhering junctions. The relative signal intensity of immunofluorescence was quantified along the yellow arrow for α-tubulin and afadin, respectively. In the orange color zone, α-tubulin was stacked on both sides of afadin-positive cell–cell contact regions (arrowheads). (D) Gel overlay analysis of cell–cell adhering junction components that bind MTs. Ex, eluate of buffer A containing 150 mM NaCl from BC-derived fraction applied SP Sepharose. E1, E2, and E3, fractions 1, 2, and 3 eluted by buffer A containing 200 mM NaCl from Ex applied Q Sepharose. α-Tub, α-tubulin. Bars, 5 µm.$

PAN of noncentrosomal MTs associate with the cell–cell junction in a side-by-side fashion. (A) SIM images of tubulin immunofluorescence in the apical and subapical planes of Eph4 cells. (B) Schematic drawing of the noncentrosomal MTs in epithelial cell sheets. In addition to the conventional noncentrosomal MTs, which are directed along the apicobasal axis, the PAN of noncentrosomal MTs appeared in the most apical plane of epithelial cell sheets. (C) SIM images of tubulin immunofluorescence in Eph4 cells. The planar apical noncentrosomal MTs are laterally associated with the cell–cell adhering junctions. The relative signal intensity of immunofluorescence was quantified along the yellow arrow for α-tubulin and afadin, respectively. In the orange color zone, α-tubulin was stacked on both sides of afadin-positive cell–cell contact regions (arrowheads). (D) Gel overlay analysis of cell–cell adhering junction components that bind MTs. Ex, eluate of buffer A containing 150 mM NaCl from BC-derived fraction applied SP Sepharose. E1, E2, and E3, fractions 1, 2, and 3 eluted by buffer A containing 200 mM NaCl from Ex applied Q Sepharose. α-Tub, α-tubulin. Bars, 5 µm.