Differentiated keratinocytes never contact the ECM while restoring the physiological epidermal differentiation pattern. Histological sections of representative EETs, homeostatic wound model regions (H) and normal human skin (NH) 24, 48, and 72 h after wounding. The sections show a double fluorescent staining of the differentiation markers cytokeratin 10 (CK10), involucrin (IVL), and filaggrin (Fil) stained with Alexa Fluor 594 in combination with CK14 (Alexa Fluor 488) as a marker for undifferentiated, basal keratinocytes. Arrowheads indicate the wound margin. Broken lines denote dermal–epidermal junction. Bar, 300 µm.