Midbody microtubules are not required for membrane shedding, ESCRT recruitment, or midbody ring release. (A) Central plane confocal images showing membrane shedding (white arrowheads) at the cell–cell boundary in a PRC1^spd-1(RNAi) embryo (n = 9 embryos) expressing an mCherry-tagged plasma membrane probe and GFP–Aurora B^AIR-2. Times are relative to anaphase of the second division. (B) Central plane confocal images showing midbody ring release in a PRC1^spd-1(RNAi) embryo expressing the midbody ring markers Myosin II^NMY-2–GFP (n = 8 embryos), GFP–CYK-7 (n = 10 embryos), or GFP-septin^UNC-59 (n = 5 embryos) along with the mCherry-tagged plasma membrane probe and mCherry-histone. Times are relative to anaphase of the second division. Midbody rings are highlighted before (yellow arrows) and after (green arrows) release from the cell–cell junction. (C) Graphs plotting the mean onset of membrane shedding (top) and midbody ring release (bottom) for control and PRC1^spd-1(RNAi) embryos. Error bars are the SDs. (D) Central plane confocal images of control (n = 6 embryos) and PRC1^spd-1(RNAi) (n = 7 embryos) embryos expressing GFP–ESCRT-I^MVB-12. Times are relative to anaphase of the second division. Dashed yellow lines mark the cell boundaries. Images are scaled equivalently. White boxes on the low magnification images in A and B mark the location of the region shown at higher magnification in the adjacent images. Bars, 5 µm.