Sticky’s essential role during MR formation is as a scaffold. (A) Anti-Sticky (Sti) immunoblot of cell lysates from indicated cell lines after Sticky 3′ UTR dsRNA or LacI control dsRNA incubation, with anti-tubulin blot as a loading control. WT, wild type. (B) Timing of abscission or furrow regression (failure) of cells coexpressing Anillin-GFP and Sticky-mCh or Sticky-KD-mCh, treated for 3–5 d with or without Sticky 3′ UTR dsRNAs. Data are from two independent experiments. (C and D) Time-lapse sequence of a cell coexpressing Anillin-GFP and Sticky-mCh (C) or Sticky-KD-mCh (D). Separated channels of the boxed regions are shown magnified at the bottom. (E) Time-lapse sequence of a cell coexpressing Anillin (Anil)-GFP and Sticky-KD-mCh after depletion of endogenous Sticky. Times are given in hours, minutes, and seconds. Bars, 5 µm. See also Videos 9 and 10.