Sticky acts with the N terminus of Anillin to promote both MR formation and CR stability. (A and B) Time-lapse sequence of cells expressing mCh-tubulin and Anillin-ΔN-GFP (A) or Anillin-ΔC-GFP (B) after a ∼30-h incubation with Sticky dsRNAs (63× objective and 2 × 2 camera binning). (C) Quantification from time-lapse records of failed attempts at cytokinesis that form internal Anillin-ΔC-FP–positive MR-like structures after incubation with the indicated dsRNAs. Data are from two independent experiments. (D and E) Confocal images of cells coexpressing Sticky-mCh and Anillin-ΔC-GFP (D) or Anillin-ΔN-GFP (E) after endogenous Anillin depletion. Dotted line in D marks the cell boundary (63× objective and no camera binning). (F) Time-lapse sequence of a cell expressing Anillin-ΔC after codepletion of Sticky and Anillin. (G) Time-lapse sequence of a cell expressing Anillin-GFP after codepletion of Pnut (144–162-h RNAi) and Sticky (30–48-h RNAi), captured with a 63× objective with 2 × 2 camera binning. Closed arrowheads mark a presumed MR remnant from a previous division. Open arrowheads mark failing MR from the current division attempt. (H) Quantification from time-lapse records (40× objective and 2 × 2 camera binning) of the percentage of Anillin-GFP and Anillin-ΔC-GFP cells displaying oscillating furrows during CR closure after incubation with the indicated dsRNAs (n > 78 per condition from two independent experiments). (I) Quantification from time-lapse records (40× objective and 2 × 2 camera binning) of attempts at cytokinesis that result in Anillin-GFP–positive MR structures after incubation with indicated dsRNAs (n > 45 per condition, from two independent experiments). Times are given in hours, minutes, and seconds. Bars, 5 µm. See also Video 8.