Proper maturation of the MR requires septin-dependent removal of Anillin via its C-terminal PHD. (A) Frequency of extrusion from time-lapse sequences of Anillin-ΔPH-GFP and of Anillin-GFP after 8-d Pnut RNAi. (B) Anti-Pnut immunoblot of S2 cell lysates serially diluted after LacI control dsRNA incubation or after Pnut dsRNA incubation; anti-tubulin is the loading control. (C) Time-lapse sequences of cells expressing Anillin-ΔPH-GFP depleted of endogenous Anillin. (D) Time-lapse sequence of an Anillin-GFP–expressing cell after 8-d Pnut RNAi. (E) Representative images of age-matched MR structures from Anillin-GFP or Anillin-ΔPH-GFP cells treated with the indicated dsRNAs. (F) Volume measurements of the nascent MR of cells expressing Anillin-ΔPH-GFP, Anillin-ΔC-GFP, or Anillin-GFP treated with the indicated dsRNAs, from the end of furrowing, normalized at each time point to equivalently aged Anillin-GFP controls (n = 10 per condition from two independent experiments). (G) Timing of abscission (abscis.) or furrow regression (fail) of Anillin-GFP cells treated for 7–9 d with LacI (control, n = 30) or Pnut dsRNAs (n = 75) and of Anillin-ΔPH-GFP cells treated for 3 d with LacI (control, n = 30) or Anillin dsRNAs (n = 75). Mean values are shown; data are from a single representative experiment out of three repeats. Times are given in hours, minutes, and seconds. Bars: (C and D) 5 µm; (E) 1 µm.