Removal of Anillin from the nascent MR is mediated via its C-terminal domains, whereas retention requires its N-terminal domains. (A) Domain organization of Anillin and truncation mutant analyses for their localization to the nascent MR, their ability to be extruded and shed, and their retention at the mature MR. Myo, myosin; Act, actin; NTD, N-terminal domain; Sep, septin; C, C terminus; N, N terminus. (B) Time-lapse sequence of a cell coexpressing Anillin-ΔC-GFP and Anillin-ΔN+CD-mCh. Open arrowheads mark shed material, and closed arrowheads mark the mature MR. Separated channels and magnified images of the boxed regions are shown at the bottom. Times are given in hours, minutes, and seconds. Bars, 5 µm. See also Video 5.