Figure 2.
Figure 2. Reconstitution of centriole appendages by Odf2 deletion constructs. (A) Electron micrographs showing distal appendages (DAs) and/or subdistal appendages (SAs) on centrioles. Thin-section electron microscopic images show cross sections (Cross) and longitudinal sections (Longitudinal) of centrioles. More than five samples were analyzed in each case. Tomography, UHVEMT images of centrioles; DAs, blue arrows; SAs, red arrowheads; WT, (DA+SA+) centriole in WT F9 cells; Δ4/5, (DA+SA−) centriole in Δ4/5 construct–expressing Odf2-KO F9 cells; Δ6/7, (DA+SA+) centriole in Δ6/7 construct–expressing Odf2-KO F9 cells; KO, (DA−SA−) centriole; TFs, blue arrows; BF, red arrowheads. Bars, 0.2 µm. Insets, schematic drawings of electron microscopic images of centrioles. (B) Summary of findings on the reconstitution of the basal body/centriole appendages.

Reconstitution of centriole appendages by Odf2 deletion constructs. (A) Electron micrographs showing distal appendages (DAs) and/or subdistal appendages (SAs) on centrioles. Thin-section electron microscopic images show cross sections (Cross) and longitudinal sections (Longitudinal) of centrioles. More than five samples were analyzed in each case. Tomography, UHVEMT images of centrioles; DAs, blue arrows; SAs, red arrowheads; WT, (DA+SA+) centriole in WT F9 cells; Δ4/5, (DA+SA−) centriole in Δ4/5 construct–expressing Odf2-KO F9 cells; Δ6/7, (DA+SA+) centriole in Δ6/7 construct–expressing Odf2-KO F9 cells; KO, (DA−SA−) centriole; TFs, blue arrows; BF, red arrowheads. Bars, 0.2 µm. Insets, schematic drawings of electron microscopic images of centrioles. (B) Summary of findings on the reconstitution of the basal body/centriole appendages.

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