Odf2 gene sequences required to generate cilia and reconstitute the appendages of ciliary basal bodies. (A) Schematic representation of the Odf2 deletion constructs, which were transfected into Odf2-KO F9 cells. LZ, leucine zipper motif. (B) Immunofluorescence for GFP (Odf2), γ-tubulin (centrioles/basal bodies), and acetylated tubulin (primary cilia) to examine the generation of cilia. Bars, 1 µm. (C) Percentage of cilia on centrioles in cells expressing the indicated construct (n > 100 in more than three independent experiments). (D) Electron micrographs showing transition fibers (TFs) and/or basal feet (BF) on ciliary basal bodies. Thin-section electron microscopic images show cross sections (Cross) and longitudinal sections (Longitudinal) of primary cilia. Tomography, UHVEMT images of basal bodies; TFs, blue arrows; BF, red arrowheads; WT, (TF+BF+) basal bodies in WT F9 cells; Δ4/5, (TF+BF−) basal bodies in Δ4/5 construct–expressing Odf2-KO F9 cells; Δ6/7, (TF+BF+) basal bodies in Δ6/7 construct–expressing Odf2-KO F9 cells. Bars, 0.2 µm. More than five samples were analyzed in each case. The cross section electron micrographs in the top two rows are the same images as in the top two rows of Fig. S3 A, where they are annotated for TFs and BF. Insets, schematic drawings of electron microscopic images of basal bodies.