Immunolocalization of histone H3 isoforms and Topo IIα in mitotic M. muntjak chromosomes. M. muntjak cells stained with anti-GFP antibody to recognize GFP–Topo IIα and costained with antibodies against H3K27me1 (A), H3K27me3 (B), or H3S28p (C). H3K27me1 and H3K27me3 are distributed throughout chromosome arms and partially colocalize with Topo IIα. H3S28p localizes peripherally to Topo IIα. Flattened z stacks are shown. Bar, 10 µm. The full z-series of C is shown in Video 3. DAPI, DNA stain. Images on the right show enlarged segments of the boxed regions (bars, 1.25 µm).